We have developed many transgenic animals which are useful for the study of neural circuitry.
1) Orexin/EGFP mice (Tg): Orexin neurons exclusively express enhanced green fluorescent protein (EGFP) under control of human prepro-orexin promoter (3.2 kb).
Related paper: Yamanaka et al., Neuron 2003, Muraki et al., J Neurosci 2004, Yamanaka et al., J Neurosci 2010
Available from RIKEN BRC
2) Orexin/Arch mice (Tg): Orexin neurons exclusively express Arch under control of human prepro-orexin promoter (3.2 kb).
Related paper: Williams et al., J Neurosci 2019
Available from RIKEN BRC RBRC05845 B6D2-Tg(hOX-Arch/EGFP)1Ahky
3) Orexin-Flippase mice (KI): Orexin neurons exclusively express Flippase recombinase and EGFP. Flippase-2A-EGFP sequence is knocked in prepro-orexin gene allele.
Related paper: Chowdhury et al., eLife 2019
Available from RIKEN BRC RBRC11555 C57BL/6-Hcrt
4) Orexin-Cre mice (Tg): Orexin neurons exclusively express Cre recombinase and EGFP. Cre-2A-EGFP sequence is randomly inserted into the genome.
Related paper: Inutsuka et al., Neuropharmacology 2014
Available from RIKEN BRC RBRC06806 C57BL/6-Tg(hOX-EGFP,-cre)1Ahky
5) Orexin-tTA mice (Tg): Orexin neurons exclusively express tetracycline transactivator (tTA: Tet off) under control of human prepro-orexin promoter (3.2 kb).
Related paper: Tabuchi et al., Sleep 2013
Available from RIKEN RBRC05841 C57BL/6-Tg(hOX-tTA)1Ahky
6) MCH-tTA mice (Tg): Melanin concentrating hormone (MCH) neurons exclusively express tetracycline transactivator (tTA: Tet off) under control of mouse pro-MCH promoter.
Related paper: Tsunematsu et al., J Neurosci 2014, Izawa et al., Science 2019
Available from RIKEN BRC RBRC05844 C57BL/6-Tg(Pmch-tTA)BAhky
7) Tph2-tTA mice (Tg): Serotonergic neurons exclusively express tetracycline transactivator (tTA: Tet off) under control of mouse Tph2 promoter (BAC Tg).
Related paper: Miyazaki et al., Current Biol 2014, Miyazaki et al., Nature Commun 2018, Takahashi et al., Nature Commun 2022.
Available from RIKEN BRC RBRC05846 B6.Cg-Tg(Tph2-tTA)1Ahky
8) DbH-tTA mice (Tg): Noradrenergic neurons exclusively express tetracycline transactivator (tTA: Tet off) under control of mouse dopamine beta hydroxylase promoter (BAC Tg).
Related paper: not yet published
Available from Akihiro Yamanaka
9) TetO ChR2 (ET/TC) (semi KI): TetO ChR2(ET/TC)::GFP was semi-KI in beta actin allele in BAC. BAC is randomly integrated into the genome. Channelrhodopsin2(ET/EC) is exclusively expressed in neurons expressing tTA.
Related paper: Tsunematsu et al., J Neurosci 2014, Izawa et al., Science 2019
Available from RIKEN BRC RBRC05843 B6.Cg-Actb
10) TetO ChR2 (C128S)) (semi KI): TetO ChR2(C128S)::GFP was semi-KI in beta actin allele in BAC. BAC is randomly integrated into the genome. Channelrhodopsin2(C128S) is exclusively expressed in neurons expressing tTA.
Related paper: Tanaka et al., Cell Reports 2012, Miyazaki et al., Current Biol 2014, Miyazaki et al., Nature Commun 2018, Ohmura et al., International J Neuropsychopharmacol 2014
Available from RIKEN BRC RBRC05454 B6;129B6(Cg)-Actb
11) TetO ArchT (semi KI): TetO ArchT::GFP was semi-KI in beta actin allele in BAC. BAC is randomly integrated into the genome. ArchT is exclusively expressed in neurons expressing tTA.
Related paper: Tsunematsu et al., Behav Brain Res 2013, Izawa et al, Science 2019
Available from RIKEN BRC RBRC05842 C57BL/6-Tg(tetO-ArchT/EGFP)1Ahky
